# Selection guide

# Surf 100Å

1.7 - 2.5 - 3.0 - 3.5 - 5.0 - 10.0 - 15.0µm Surf 100Å - 330 m²/g spherical high purity silica-gel

for small organic, acidic, neutral, basic molecules analysis < 1500 MW

7 C18 selectivity,
to answer challenging analysis from highly polar to non-polar samples

Completed by,

C30, C8AQ, C8PTF, C8P, C8, C8T, C8EX, C4, CN
Hilic
Phenyl, Phenyl-Hexyl,
NH2, SCX,
Diol, Silica

 
Our Surf 100Å silica-gel perfectly spherical has a very high purity at > 99.99% SiO₂. his makes it a very inert product ideal across the board for Bio & Pharmaceutical applications. The narrow distribution of porosity & particle (~ mono-disperse) is an important feature of our silica, and almost all pores are open-ended, creating several advantages:

• Great permeability
• Higher accessibility to bonded surface
• Narrow peaks shape
• Easier to pack
• Better packing compacity

The pore volume is one of the lower, meaning lower dead volume in the column. The T0 is eluted earlier, finally you achieve a shorter runtime for the method while keeping the resolution.
*(at identical selectivity and for same particle size)

Surf C18 Selectivity guide & positioning

# Shaper Hybrid 

Analysis of Basic Drugs under basic conditions

• 130Å - 185 m²/g Organic / Inorganic balance
• Strong pH stability 1 up to 12
• High mechanical strength = longer column lifetime.
• Anhydrous gas phase multiple end-capping technology
• Excellent choice for method development of new analysis up to preparative scale.

Our Shaper Hybrid silica backbone has been developed through silicone hydrolysis polycondensation creating an optimum organic / inorganic balance and homogeneous pore structure that provides a strong pH stability under both acidic and basic conditions (1-to-12) and a high mechanical strength leading to a longer column lifetime.

9 stationary phases are available:

C18 :

USP L1, Hydrophobic mechanism of retention design for Acids, Bases and Neutrals compounds analysis. pH stability: 1 to 12.

C18 Polar :

USP L60, Hydrophobic mechanism of retention design for mid-polar compounds analysis. Compatible with 100% H₂O mobile phase.
pH stability: 1 to 11

C18 AX :

USP L78, Greater retention of polar acidic compounds with different selectivity from C18 chromatography columns. Under acidic conditions, the retention of alkaline compounds is weakened.

C8 :

USP L7, Shorter retention vs. C18 phase for late eluted compounds. pH stability: 1 to 12

Phenyl-Hexyl :

USP L11, Orthogonal selectivity to C18, design for polar aromatic compounds analysis & moderately polar analytes. pH stability: 1 to 12

Penta-FP :

USP L43, Pi-Pi interactions, dipole, hydrogen bonding, and ionic interactions to perform highly polar compound

Hilic :

USP L20, design for highly polar compounds (Log P < 0.5) separation – that cannot be done on C18 - under highly % of organic

HYB - Amide
L68
pH stability 2 - 11
Temp. stability : 90°C

Separation & Analysis of strong polar compounds in HILIC mode, which are not retained by C18.
Glycan analysis.

 

Hilic – Zwitterionic
L114
pH stability 2 - 10
Temp. stability : 60°C

Separation of Sugars, Organic Acids, Metabolites & amino acids.
Enhance the retention of polar compounds while providing a unique selectivity distinct from HILIC and Amide columns.

# Shaper Hybrid Surface + 

Analysis of Basic Drugs under acidic conditions

• 130Å - 185 m²/g Organic / Inorganic balance
• Strong pH stability 1 – 12
• Surface + technology = Positively Charged Surface

Analysis of amitriptyline under Acetonitrile / Phosphate buffer pH:2.9

3 Stationary phases are available :

C18 :

USP L1, Hydrophobic mechanism of retention design for Acids, Bases and Neutrals compounds analysis.

Phenyl-Hexyl :

USP L11, Orthogonal selectivity to C18, design for polar aromatic compounds analysis & moderately polar analytes.

Penta-FP :

USP L43, Pi-Pi interactions, dipole, hydrogen bonding, and ionic interactions to perform highly polar compound

# Shaper High Mechanical Stength

Methods transfer HPLC => UHPLC conditions

• 100Å - 230 m²/g Organic / Inorganic balance
• High Mechanical Strength
• Longer lifetime
• Transferring HPLC methods to U-HPLC
• pH stability 1-to-8

# Shaper Hybrid <=> Partially Porous 

2 innovative technologies, a unique particle

• Nonporous, impermeable silica center
• surrounded by a porous Hybrid shell layer of 0,4 µm.

2 stationary phases are available :

C18 Ultra Inert :

USP L1, Hydrophobic mechanism of retention design for Acids, Bases and Neutrals compounds analysis. pH stability: 1 to 12

Phenyl-Hexyl :

USP L11, Orthogonal selectivity to C18, design for Polar aromatic compounds analysis. pH stability: 1 to 12

Shaper PP 2.6 µm C18UI, 150 x 3.0 mm
60/40 Acetonitrile/Water @1.5 ml/min

1. Uracil (0.015 mg/ml)
2. Phenol (0.7 mg/ml)

# Shaper Partially Porous

• Nonporous, impermeable silica center
• surrounded by a porous shell layer of 0,4 µm.

100Å - 115 m²/g
2.1, 3.0 & 4.6 mm id
50, 100, 150 & 250 mm length

Excellent choice to optimize classical validated methods

6 stationary phases are available : 

C4 :

USP L26, Lower hydrophobicity than C8 and C18, faster separations for very hydrophobic compounds while increased retention for highly polar/hydrophilic compounds.

Bi-Phenyl :

USP L11, Greater selectivity for hard to resolve compounds or early eluted using a C18. Good separation of aromatic & moderately polar analytes

Penta FP :

USP L43, Pi-Pi interactions, dipole, hydrogen bonding, and ionic interactions to perform highly polar compound analysis, can be used in Hilic mode.

Amide :

USP L68, Separation & Analysis of strong polar compounds in Hilic mode, which are not retained by C18. Alcohols, Phenols and Catechins, Glycan analysis.

Hilic-DH :

USP L20, design for mid & low polar compounds (Log P < 0.5) separation - that cannot be done on C18 - under highly % of organic

Hilic-SI :

USP L3, design for mid polar compounds (Log P < 0.5) separation - that cannot be done on C18 - under highly % of organic

# SelectChem

• SelectChem Polar Embedded C18
  USP L60
  120Å - 300 m²/g ultrapure, inert silica
  3.5 & 5.0 µm
  Tri-functional C18 (20%) end-capped + amide inserted polar group
  Stable pH: 1 – 8

• SelectChem C18 Shield
  USP L1
  120Å - 300 m²/g ultrapure, inert silica
  3.5 & 5.0 µm
  mono-functional C18 (17%) end-capped + carbamate inserted group
  Stable pH: 2 – 8

• SelectChem 200 C30W
  USP L62
  200Å - 140 m²/g ultrapure, inert silica
  3.5 & 5.0 µm
  C30 (14%) double end-capped
  Stable pH: 1 – 8

Polar substances such as sugar, nucleic acid, Fat-soluble substances such as vitamin E, vitamin K1 and carotenoids

# Surf Bio 150 - 200 - 300 Å

We produce Surf bio-version for peptides, polypeptides, oligo-nucleotides analysis under reverse phase conditions: