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Technical Notes

 As a long time specialist in Chromatography, imChem's goal is to share its vast knowledge with its users under useful notes and tips to improve your chromatography. 


  • hplc    Regeneration of reversed phase HPLC columns  »  (0.17 MB)
    One of the most frequent questions we are asked concerns the best process for washing and regenerating HPLC columns...
  • hplc    Split/double peaks in HPLC  »  (0.17 MB)
    The causes usually lie in the fact that the pH of the mobile phase is too close to the pKa of a functional group of the...
  • hplc    Erratic pressures in the HPLC system  »  (0.16 MB)
    More than once we have been told by HPLC users, that there is an erratic pressure on their system, usually thinking that this symptom comes from the column...
  • hplc    Filtration in HPLC  »  (0.17 MB)
    One of the crucial points for the longevity of columns and HPLC systems is the filtration of whatever we insert into an HPLC system, whether samples or eluents...
  • hplc    HPLC buffer precipitation  »  (0.16 MB)
    One of the recurring problems that most questions we receive is about high pressures in an HPLC column. After asking some questions and detecting some obvious symptoms...
  • hplc-usp    HPLC USP – Selection Guide  »  (0.30 MB)
    7 pages of USP phase/columns selection table...
  • hplc    Normal phase HPLC column regeneration  »  (0.17 MB)
    Here are some general procedures to follow to regenerate normal phase columns...
  • hplc    Pressure drop in the HPLC system  »  (0.17 MB)
    Virtually any HPLC user knows the reasons for a pressure increase in the HPLC system, but what can be done if a pressure drop occurs?...
  • hplc    Reduction of solvent consumption in HPLC  »  (0.17 MB)
    With the past shortage of Acetonitrile, the consequent increase in its price has had a significant impact on the cost of each HPLC analysis and...
  • gc    How to degas your eluents  »  (0.24 MB)
    The degassing of eluents in HPLC is an important step to ensure a high sensitivity and detection limit in your HPLC method. The existence of excess oxygen dissolved in the eluent promotes the formation of small bubbles that...
  • gc tips    Ferrule Choice  »  (0.27 MB)
    There are frequent doubts concerning the different materials used to manufacture ferrules for the GC columns. Despite having the same dimensions and the same way of installing...
  • gc tips    Conditionning of GC capillary columns  »  (0.16 MB)
    One of the most recurring questions concerns the proper conditioning of the GC columns, so following you will find our recommendations for proper conditioning...
  • gc    Separation of late peaks in GC  »  (0.17 MB)
    One of the most frequently asked questions in GC is how to improve the separation of late peaks in GC. Most of the time...
  • gc    Separation of peaks with low GC retention  »  (0.17 MB)
    Separation of peaks with low GC retention ?...


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